Using Natural-Product-Based Treatments, Such as Polymer loaded with Ginger for the Management of Osteochondral Disorder

Ben Salah, Ghada

doi:10.22068/ijmse.3429

Volume 21, Issue 3 (September 2024) IJMSE 2024, 21(3): 106-115 | Back to browse issues page

‎ 10.22068/ijmse.3429

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Ben Salah G. Using Natural-Product-Based Treatments, Such as Polymer loaded with Ginger for the Management of Osteochondral Disorder. IJMSE 2024; 21 (3) :106-115
URL: http://ijmse.iust.ac.ir/article-1-3429-en.html

Using Natural-Product-Based Treatments, Such as Polymer loaded with Ginger for the Management of Osteochondral Disorder

Ghada Ben Salah

Abstract: (11081 Views)

This study reported the biological changes occurring after γ-irradiation of in vivo rat model and the osteochondral protective effect of Gelatine-Chitosan-Ginger (GEL-CH-GING). The results showed that Electron Paramagnetic Resonance (EPR) Spectroscopy of GEL-CH-GING showed two paramagnetic centres which correspond to g=2.19 and g= 2.002. The Fourier transform infrared spectroscopy (FTIR) analyses revealed an increase in peak intensity at C–H chains, as well as, C=O carbonyl groups. The X-ray diffraction (XRD) analysis showed no change of crystallinity. After gamma ray exposure, the rat groups have received an osteochondral defect and then were treated with GEL-CH-GING composite. Sixty days post-surgery, a significant reduction in thiobarbituric acid-reactive compounds (TBARs) was seen when compared to non-implanted rat group. Concerning oxidative stress status, GEL-CH-GIN significantly improved Superoxide Dismutase (SOD) 76 nmol/l, Catalase (CAT) 0.79 nmol/l, and Glutathione Peroxidase (GPx) 1.77 nmol/l activities in osteochondral tissue. Regarding the histomorphometric parameters of cartilaginous tissue (nCg.Th, µm), (cCg.Th, µm), (Cg.Th, µm), irradiated-GEL-CH-GIN group showed a significant increase as compared to irradiated group with 116, 74 and 188 µm, respectively (p<0.01). The microanalysis showed a high percentage of O and C in the regenertaed osteochondral tissue and indicated the deposition of novel collagen matrix. The biomechanical behaviour showed a significantly enhanced hardness measurement (1.73±0 .029 VH, p<0.05) when compared with that of irradiated group
Biochemical markers suggested an osteocartilage repair capacity. In fact, the levels of IL-1β, IL-6, TNF-α and VEGF in the implanted rat with GEL-CH-GING composite exhibited 51±3.48, 30.05±5.18, 65.12±4.33 and 40.42±3.32 ng/l, respectively. Our findings suggested that GEL-CH-GING composite might have promising potential applications for cartilage healing.

Keywords: Ginger, γ-irradiation, Biomaterial, oxidative stress, osteochondral defect