en Research Paper Research Paper <span style="font-size:12pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Cambria,serif"><span style="font-size:11.0pt"><span style="background:white"><span style="line-height:200%"><span calibri="" style="font-family:"><span style="color:black">This study explores the fabrication, structural analysis, and cytocompatibility of cobalt-doped bioactive glass scaffolds for potential applications in bone tissue engineering. A specific glass composition modified from Hench&#39;s original formulation was melted, quenched, and ground to an average particle size of 10 &mu;m. The resulting amorphous powder underwent controlled sintering to form green bodies and was extensively characterized using simultaneous differential thermal analysis (DTA), Raman spectroscopy, and Fourier Transform Infrared analysis (FTIR). After mixing with a resin and a dispersant, the composite was used in digital light processing (DLP) 3D printing to construct scaffolds with interconnected macropores. Thermal post-treatment of 3D printed scaffolds, including debinding (Removing the binder that used for shaping) and sintering, was optimized based on thermogravimetric analysis (TG) and the microstructure was examined using FE-SEM and XRD. In vitro bioactivity was assessed by immersion in simulated body fluid (SBF), while cytocompatibility with MC3T3 cells was evaluated through SEM following a series of ethanol dehydrations. The study validates the fabrication of bioactive glass scaffolds with recognized structural and morphological properties, establishing the effects of cobalt doping on glass behavior and its implications for tissue engineering scaffolds. Results show, Low cobalt levels modify the glass network and reduce its Tg to 529 ^oC, while higher concentrations enhance the structure in point of its connectivity. XRD results shows all prepared glasses are amorphous nature, and DTA suggests a concentration-dependent Tg relationship. Spectroscopy indicates potential Si-O-Co bonding and effects on SiO2 polymerization. Cobalt&#39;s nucleating role promotes crystalline phases, enhancing bioactivity seen in rapid CHA layer formation in SBF, advancing the prospects for bone tissue engineering materials.</span></span></span></span></span></span></span></span></span></span> Tissue engineering,bioactive glass-ceramic,scaffold,glass structure 1 23 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-3897-1&slc_lang=en&sid=1 Ahad Saeidi ahadsaeidi@gmail.com 1800319475328460020108 1800319475328460020108 No Department of Ceramic, Materials and Energy Research Center, Karaj, Iran Sara Banijamali banijamali@merc.ac.ir 1800319475328460020109 1800319475328460020109 Yes Department of Ceramic, Materials and Energy Research Center, Karaj, Iran Mojgan Heydari m.heydari@merc.ac.ir 1800319475328460020110 1800319475328460020110 No Department of Nano Technology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran