en Research Paper Research Paper <span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Biogenic synthesis of papain-conjugated copper metallic Nanoparticles and their antibacterial and antifungal activities Papain metallic conjugated nanoparticles (Papain-CuNPs) were synthesised using Papain and CuSO4.5H₂O. Papain-CuNPs were characterized using UV-visible spectroscopy, FT-IR, HR-TEM, XRD, FE-SEM, zeta potential, and a zeta sizer. The antibacterial activity of papain-CuNPs against human infectious microorganisms (<i>Citrobacter spp</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>) was investigated. The mechanism of action of papain-CuNPs was evaluated using FE-SEM and HRTM.<i> </i>UV spectroscopy confirmed the plasma resonance (SPR) at 679 nm, which indicated the formation of papain-CuNPs. The FT-IR spectrum absorbance peaks at 3927, 3865, 3842, 3363, 2978, and 2900 cm^-1 indicate the presence of O-H and N-H of the secondary amine, and peaks at 1643 and 1572 cm^-1 represent C=O functional groups in Papain-CuNPs. EDAX analysis confirmed the presence of copper in the papain-CuNPs. The zeta potential (-42.6 mV) and zeta size (99.66 d. nm) confirmed the stability and size of the nanoparticles. XRD confirmed the crystalline nature of the papain-CuNPs. FE-SEM and HRTM showed an oval structure, and the nano particles&#39; 16.71244&ndash;34.84793 nm. <a name="_Hlk130067570">The synthesized papain-NPs showed significant antibacterial activity against clinical <i>P. aeruginosa</i> (15 mm). MIC 125 &micro;g/ml) showed bactericidal activity against <i>P. aeruginosa</i> and the mechanism of action of Papain-NPs was confirmed using an electron microscope by observing cell damage and cell shrinking</a>. Papain-CuNPs have significant antibacterial activity and are thus used in the treatment of <i>P. aeruginosa</i> infections</span></span></span> Papain,Copper sulphate,Nanoparticles,SE-SEM,HRTEM 125 134 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-5284-1&slc_lang=en&sid=1 Muddukrishnaiah Kotakonda krishna123muddu@gmail.com 1800319475328460019281 1800319475328460019281 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malapuram District, Kerala, India. Sajisha V.S sajishair@yahoo.co.in 1800319475328460019282 1800319475328460019282 No Department of Pharmaceutics, JDT Islam College of pharmacy, Jamiath Hill, Marikunnu P O, Calicut, Kerala, India. Aiswarya G Aiswaryabasil2012@gmail.com 1800319475328460019283 1800319475328460019283 No Department of Pharmaceutics, JDT Islam College of pharmacy, Jamiath Hill, Marikunnu P O, Calicut, Kerala, India. Safeela Nasrin Pakkiyan Safeelanasrin2018@gmail.com 1800319475328460019284 1800319475328460019284 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malapuram District, Kerala, India. Najamol A Alungal Najaalungal109@gmail.com 1800319475328460019285 1800319475328460019285 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Mayoora Kiliyankandi K Mayooravijay314@gmail.com 1800319475328460019286 1800319475328460019286 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Divya Thekke Kareth divyaprajith@gmail.com 1800319475328460019287 1800319475328460019287 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Naheeda Ashraf Verali Parambil naheedavp@gmail.com 1800319475328460019288 1800319475328460019288 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Saranya Sasi Mohan Saranyasmohans66@gmail.com 1800319475328460019289 1800319475328460019289 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Renjini Anil Sheeba 2017renjinias@gmail.com 1800319475328460019290 1800319475328460019290 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Sarika Puthiya Veettil Sarikapv63@gmail.com 1800319475328460019291 1800319475328460019291 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Dhanish Joseph dhanish@nirmalacp.orgs 1800319475328460019292 1800319475328460019292 No Department of Pharmaceutics, Nirmala College of Pharmacy, Muvattupuzha P.O Ernakulam District Kerala, India Nishad Kakkattummal nishadcvr@gmail.com 1800319475328460019293 1800319475328460019293 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Afsal Bin Haleem MP Afsalhaleem2@gmail.com 1800319475328460019294 1800319475328460019294 No Department of Pharmacy, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, Kerala, India. Safeera Mayyeri Safeera67067@gmail.com 1800319475328460019295 1800319475328460019295 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Thasneem Chemban Koyilott thasneemck@gmail.com 1800319475328460019296 1800319475328460019296 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Nasiya Nalakath nasiyalukman@gmail.com 1800319475328460019297 1800319475328460019297 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Samuel Thavamani B samtmani78@gmail.com 1800319475328460019298 1800319475328460019298 No Department of Pharmacognosy, Ahalia School of Pharmacy, Ahalia Campus, Kozhipara, Palakkad, Kerala, India Famila Rani J familajacob28@gmail.com 1800319475328460019299 1800319475328460019299 No Department of Pharmaceutical Chemistry, JKKN College of Pharmacy, Komarapalayam. Salem, Tamil Nadu Aruna Periyasamy aruna01818@gmail.com 1800319475328460019300 1800319475328460019300 No Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Chidambaram Tami Nadu, India. Chellappa V Rajesh rajeshcvsky@gmail.com 1800319475328460019301 1800319475328460019301 No Department of Pharmaceutics, United College of Pharmacy, Coimbatore, Tamil Nadu, India. Rameswari Shanmugam rameswari.mpharm@gmail.com 1800319475328460019302 1800319475328460019302 No Department of Pharmaceutical Chemistry, Sree Sastha Pharmacy College, Chembarambakkam, Tamil Nadu, India. Marimuthu Poornima poorni.medicare@gmail.com 1800319475328460019303 1800319475328460019303 No Department of Pharmaceutical Chemistry, Sree Sastha Pharmacy College, Chembarambakkam, Tamil Nadu, India. Tina Raju tinarcpmht@gmail.com 1800319475328460019304 1800319475328460019304 No Department of Pharmaceutics, DM WIMS College of Pharmacy, Wayanad, Kerala, India Roshni E R roshniram19@gmail.com 1800319475328460019305 1800319475328460019305 No Department of Pharmacy, Sanjo College of Pharmaceutical Studies, Vellapara, Palakkad, Kerala, India. Sirajudheen Mukriyan Kallungal mksiraj@gmail.com 1800319475328460019306 1800319475328460019306 No Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India. Lekshmi MS Panicker Leku17@gmail.com 1800319475328460019307 1800319475328460019307 No Department of Pharmaceutics, Mar Dioscorus College of Pharmacy, Thiruvananthapuram, Kerala, India. Saranya K G sharusreejith02@gmail.com 1800319475328460019308 1800319475328460019308 No Department of Pharmaceutics, Nirmala College of Health Science, Nutmeg Gardens, Kunnappilly Post, Chalakudy, Kerala, India. Shilpa V P shilpavp24@gmail.com 1800319475328460019309 1800319475328460019309 Yes Department of Pharmacy, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram District, Kerala, India.