en Research Paper Research Paper <span style="font-size:12pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span lang="EN-US" style="font-size:11.0pt"><span style="line-height:200%"><span new="" regular="" roman="" style="font-family:" times="">Researchers are increasingly focusing on green synthesis methods for silver nanoparticles due to their cost-effectiveness and reduced environmental impact. In this study, we utilized an edible bird&#39;s nest (EBN), a valuable economic resource, as the primary material for synthesizing silver nanoparticles using only water as the solvent. Metabolite profiling of the EBN extract was conducted using LC-QTOF-MS in positive mode (ESI+), revealing the presence of lipids, glycosides, peptides, polysaccharides, and disaccharides. Upon the addition of silver nitrate to the aqueous EBN extract, noticeable color changes from transparent to brown indicated the successful formation of AgNPs. Subsequent characterization of these silver nanoparticles involved UV-Visible spectroscopy, which revealed an absorption peak at 421 nm. Further characterization was carried out using FESEM, ATR-FTIR spectroscopy, and EDX analysis. The involvement of phenolic agents, proteins, and amino acids in reducing the silver particles was confirmed. The synthesized nanoparticles exhibited a spherical shape, and a particle size ranging from 10 to 20 nm. The presence of elemental silver was confirmed by a strong, intense peak around 3 keV in the EDX spectrum. To assess their potential, the antibacterial properties of the silver nanoparticles against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> were evaluated using the agar diffusion method.</span></span></span></span></span></span></span><br> &nbsp; Aerodramus fuciphagus,antibacterial properties,green synthesis,silver nanoparticles. 108 117 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-5239-1&slc_lang=en&sid=1 FARAH Hanani ZULKIFLI farahhanani@ump.edu.my 1800319475328460020121 1800319475328460020121 Yes Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang Darul Makmur. Hamid Hazrulrizawati 1800319475328460020122 1800319475328460020122 No Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang Darul Makmur 2 Center for Advanced Intelligent Materials, Universiti Malaysia Pahang Al-Sultan Abdulla, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang Darul Makmur Fathima Shahitha Jahir Hussain 1800319475328460020123 1800319475328460020123 No The College of Arts and Sciences, University of Nizwa Initial Campus, Birkat Al Mouz, P.O. Box 33, PC 616, Nizwa, Sultanate of Oman Nur Fatini Ilyana Mohamat Johari 1800319475328460020124 1800319475328460020124 No Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang Darul Makmur 2 Center for Advanced Intelligent Materials, Universiti Malaysia Pahang Al-Sultan Abdulla, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang Darul Makmur